• Concepts for structured illumination microscopy with extended axial resolution through mirrored illumination 

      Manton, James D.; Ströhl, Florian; Fiolka, Reto; Kaminski, Clemens F.; Rees, Eric J. (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-03-20)
      Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical sectioning and to double the resolution limit both laterally and axially, but even with this the axial resolution is still worse than the lateral resolution of ...
    • OptiJ: Open-source optical projection tomography of large organ samples 

      Vallejo Ramirez, Pedro P.; Zammit, Joseph; Vanderpoorten, Oliver; Fergus, Riche; Blé, Francois-Xavier; Zhou, Xiao-Hong; Spiridon, Bogdan; Valentine, Christopher; Spasov, Simeon E.; Oluwasanya, Pelumi W.; Goodfellow, Gemma; Fantham, Marcus J.; Siddiqui, Omid; Alimagham, Farah; Robbins, Miranda; Stretton, Andrew; Simatos, Dimitrios; Hadeler, Oliver; Rees, Eric J.; Ströhl, Florian; Laine, Romain F.; Kaminski, Clemens F. (Journal article; Tidsskriftartikkel; Peer reviewed, 2019-10-30)
      The three-dimensional imaging of mesoscopic samples with Optical Projection Tomography (OPT) has become a powerful tool for biomedical phenotyping studies. OPT uses visible light to visualize the 3D morphology of large transparent samples. To enable a wider application of OPT, we present OptiJ, a low-cost, fully open-source OPT system capable of imaging large transparent specimens up to 13 mm tall ...